French Open champion Garbine Muguruza heads into Wimbledon as the second seed behind Serena Williams, her highest seeding for a Grand Slam.
Muguruza claimed victory over Williams at Roland Garros last month to put herself on the list of Grand Slam winners, having come so close at the All England Club 12 months ago.
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The Spaniard reached the final of the grass-court championship in 2015, only to lose in straight sets to Williams.
Rising stars Belinda Bencic and Madison Keys are also seeded inside the top 10, while Great Britain's Johanna Konta is the 17th seed — the first Briton seeded in the women's draw since 1984.
There is no seeding for former world No. 1 Caroline Wozniacki, though, the Dane having struggled for form following an ankle injury.
In the men's draw Novak Djokovic and Andy Murray are unsurprisingly the top two seeds, with Roger Federer third.
In-form Dominic Thiem has been named the eighth seed, with fellow Next Generation star Alexander Zverev taking the 24th seeding.
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Wimbledon begins on Monday, with the draw for 2016's third Grand Slam taking place on Friday.
Men
John Isner at 18 is the top American seed. Other Americans among the top 32: Jack Sock, 27, and Sam Querrey, 28. Canada's Milos Raonic is No. 6.
1 Novak Djokovic, Serbia
2 Andy Murray, Britain
3 Roger Federer, Switzerland
4 Stan Wawrinka, Switzerland
5 Kei Nishikori, Japan
6 Milos Raonic, Canada
7 Richard Gasquet, France
8 Dominic Thiem, Austria
9 Marin Cilic, Croatio
10 Tomas Berdych, Czech Republic
11 David Goffin, Belgium
12 Jo-Wilfried Tsonga, France
13 David Ferrer, Spain
14 Roberto Bautista Agut, Spain
15 Nick Kyrgios, Australia
16 Gilles Simon, France
17 Gael Monfils, France
18 John Isner, United States
19 Bernard Tomic, Australia
20 Kevin Anderson, South Africa
21 Philipp Kohlschreiber, Germany
22 Feliciano Lopez, Spain
23 Ivo Karlovic, Croatia
24 Alexander Zverev, Germany
25 Viktor Troicki, Serbia
26 Benoit Paire, France
27 Jack Sock, United States
28 Sam Querrey, United States
29 Pablo Cuevas, Uruguay
30 Alexandr Dolgopolov, Ukraine
31 Joao Sousa, Portugal
32 Lucas Pouille, France
Women
Behind Serena Williams at No. 1, Americans find places throughout the list of 32 seeded players. Venus Williams is No. 9, with Madison Keys No. 10. Sloane Stephens is No. 19, and Coco Vandeweghe is No. 28.
NOTE: Victoria Azarenka, who was the sixth seed, withdrew from the tournament because of a knee injury.
1. Serena Williams, United States
2. Garbine Muguruza, Spain
3. Agnieska Radwanska, Poland
4. Angelique Kerber, Germany
5. Simona Halep, Romania
6. Roberta Vinci, Italy
7. Belinda Bencic, Switzerland
8. Venus Williams, United States
9. Madison Keys, United States
10. Petra Kvitova, Czech Republic
11. Timea Bacsinszky, Switzerland
12. Carla Suarez Navarro, Spain
13. Svetlana Kuznetsova, Russia
14. Samantha Stosur, Australia
15. Karolina Pliskova, Czech Republic
16. Johanna Konta, Britain
17. Elina Svitolina, Ukraine
18. Sloane Stephens, United States
19. Dominika Cibulkova, Slovakia
20. Sara Errani, Italy
21. Anastasia Pavlyuchenkova, Russia
22. Jelena Jankovic, Serbia
23. Ana Ivanovic, Serbia
24. Barbora Strycova, Czech Republic
25. Irina-Camelia Begu, Romania
26. Kiki Bertens, Belgium
27. Coco Vandeweghe, United States
28. Lucie Safarova, Czech Republic
29. Daria Kasatkina, Russia
30. Caroline Garcia, France
31. Kristina Mladenovic, France
32. Andrea Petkovic, Germany