Gerard Pique followed a tearful Jordi Alba off the pitch as Barcelona suffered a double injury blow against Manchester City on Wednesday.
Alba complained of muscular problems while on international duty recently and appeared to aggravate a pre-existing issue in the Champions League tie at Camp Nou.
He was replaced by Lucas Digne before Pique limped off having failed to shake off the effects of a clash with David Silva, with Jeremy Mathieu coming on in central defense.
Alba's injury is a further blow for Barcelona boss Luis Enrique, who is already facing a shortage of bodies in the fullback positions.
Sergio Roberto missed the City clash with an injury and with Aleix Vidal out of favor, Javier Mascherano was forced to start at right back.