The Week 4 matchup between the Pittsburgh Steelers and the Tennessee Titans will be played later in the NFL season following further positive coronavirus tests.
The game was pushed back from Sunday to Monday or Tuesday after the Titans confirmed three players and five personnel staff had contracted COVID-19.
However, following two further positive tests in the Tennessee camp, one for a player and one among the staff, the teams will now meet later in the season.
An NFL statement released on Thursday read: "The Pittsburgh-Tennessee game scheduled for Week 4 will be rescheduled to later this season after one additional Titans player and one personnel member tested positive for COVID-19 today. An announcement of the new game date will be made shortly.
"The decision to postpone the game was made to ensure the health and safety of players, coaches and game day personnel.
"The Titans facility will remain closed and the team will continue to have no in-person activities until further notice."
Though the entire NFL preseason slate was scrapped this year, this is the first instance of a regular-season game being postponed because of the coronavirus crisis.
The Titans and Steelers have both started 3-0, two of seven NFL teams yet to lose a game through three weeks.